eBioscience™ Mouse IL-23, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 34-8231-82

100UG Mouse IL-23 Recombinant Protein Carrier-Free

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15598766

Quantity Price
1 £ 1955.6 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Accession Number NM_008352 co-expressed with AF301619
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid; 20mM Phosphate, 0.2M NaCl, pH 6, 0.22μm filtered.
Molecular Weight The heterodimer of p40, amino acids met 23-ser 355, cystine-linked to p19, amino acids leu 20-ala 196, has a predicted molecular mass of 55,616. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 60kDa protein. The DTT reduced protein migrates as 43kDa and 21kDa polypeptides.
Name IL-23
Purification Method SDS-PAGE
Purity >98%
Quantity 100μg
Recombinant Yes
Source Insect Cells
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Structural Form Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 355, accession # NM_008352 was co-expressed with mouse p19, amino acids met 1-ala 196, accession # AF301619
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Gene Symbol Interleukin-23, p40, p19
Biological Activity The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625pg/mL. This corresponds to a specific activity of greater than 1.5 x 106 Units/mg.
Product Type Recombinant Protein, Carrier-Free

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naïve T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naïve and memory T cells, as compared to IL-12.