eBioscience™ Human TNF alpha, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8329-63

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.00001kg



Disclaimers: For Research Use Only.

Product Code. 15207918

Quantity Price
1 £ 66.65 / 10µg
EU Stock 2
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Description and Specification

Specification

Purification Method SDS-PAGE
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Quantity 10μg
Cross Reactivity Human
Conjugate Unlabeled
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Purity >95%
Source E. coli
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Name TNF alpha
Validated Application FA
Accession Number HUMTNFAB
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 80pg/mL. This corresponds to a specific activity of greater than or equal to 1.3 x 107 Units/mg.
Molecular Weight 19kDa
Recombinant Yes
Regulatory Status RUO
Structural Form E. coli expressed amino acids Val 77-Leu 233 of human TNF- alpha accession # HUMTNFAB, N-terminal His-tag
For Use With (Application) Cytokine Bioassays

Human TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.