eBioscience™ Human TNF alpha, Recombinant Protein

Human Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8329-62

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15597516

Quantity Price
1 £ 54.42 / 5µg
Estimated Shipment date
from Supplier 05-06-2017
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Description and Specification


Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Conjugate Unlabeled
Cross Reactivity Human
Recombinant Yes
Name TNF alpha
Validated Application FA
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
Quantity 5μg
For Use With (Application) Cytokine Bioassays
Molecular Weight 19kDa
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 80pg/mL. This corresponds to a specific activity of greater than or equal to 1.3 x 107 Units/mg.
Regulatory Status RUO
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Accession Number HUMTNFAB
Purity >95%
Structural Form E. coli expressed amino acids Val 77-Leu 233 of human TNF- alpha accession # HUMTNFAB, N-terminal His-tag
Source E. coli
Purification Method SDS-PAGE

Human TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.