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Invitrogen™ TNF alpha Human ELISA Kit, High Sensitivity

High Sensitivity Sandwich ELISA Kit

Brand:  Invitrogen™ BMS223HS

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15521747

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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human TNF-alpha
Biotin-Conjugate anti-human TNF-alpha polyclonal antibody
Streptavidin-HRP
Human TNF-alpha Standard lyophilized, 1ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human High Sensitivity Tumor Necrosis Factor alpha (HS TNF-α) ELISA quantitates Hu HS TNF-α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS TNF-α. Principle of the method The Human HS TNF-α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
Specifications

Specifications

P01375
0.13 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
8.5%
HRP
RUO
2°C to 8°C
Human
4 hr.
0.31-20.0 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
7124
9.8%
Pre-coated 96 well plate, Wash Buffer, Sample Diluent, Stop Solution, Adhesive Plate Covers, Amplification Diluent Concentrate, Sample Diluent, Assay Buffer concentrate, SAV-HRP, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Contols
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Cachectin, Tumor necrosis factor alpha, DIF
1 hr. 20 min.
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