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Invitrogen™ TIE2 Human ELISA Kit

For the quantitative detection of human TIE-2.

Brand:  Invitrogen™ BMS2042

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15581557

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Includes: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human TIE-2
Biotin-Conjugate anti-human TIE-2 polyclonal antibody
Streptavidin-HRP
Human TIE-2 Standard lyophilized, 20ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human Tyrosine Kinase Receptor (Hu TIE-2) ELISA quantitates Hu TIE-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIE-2. Principle of the method The Human TIE-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TEK is a tyrosine-protein kinase that acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4. It regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, and cell spreading. TEK has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. It is required for normal angiogenesis, heart development during embryogenesis, and post-natal hematopoiesis. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1, and TIE1. Mutations in the gene can result in dominantly inherited venous malformations and glaucoma 3.
Specifications

Specifications

Q02763
0.03 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD202B,TIE-2,TIE2,VMCM,VMCM1
7.1%
HRP
RUO
2°C to 8°C
Human
21 hr. 10 min.
0.16-10.0 ng/mL
Biotin
Plasma, Serum, Supernatant
ELISA
7010
6.3%
Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Assay Buffer concentrate, SAV-HRP, SAV-HRP, Wash Buffer, Stop Solution, Stop Solution, Controls
96 Tests
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
CD202b antigen, VMCM1, TEK
1 hr. 20 min.
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