Invitrogen™ Thrombospondin 1 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHTHBS1

Product Code. 15455143

Description

The Human Thrombospondin 1 ELISA quantitates Hu Thrombospondin 1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Thrombospondin 1. Principle of the method The Human Thrombospondin 1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Specifications

• Accession Number: P07996
• Assay Sensitivity: 6 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7057
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Cardiovascular Research
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 8.19-2000 ng/mL
• Calibration Range: 6ng/mL to 2000ng/mL
• Conjugate: Biotin
• Protein Family: Growth Factors & Receptors
• Specificity: This ELISA pair antibody detects human Thrombospondin-1. It shows less than 5% cross-reactivity with recombinant human Thrombospondin-2 and recombinant human Thrombospondin-4.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: THBS,THBS-1,TSP,TSP-1,TSP1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
• Synonym: THBS, THBS-1, TSP, TSP-1, TSP1
• Test Time: 1 hr. 20 min.