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Invitrogen™ TGF alpha Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHTGFA

336.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.00400kg

Product Code. 15415143

  • £504.00 / 1 set
Estimated Shipment: 23-04-2024
to see stock.
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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Human Transforming Growth Factor (TGFα) ELISA quantitates Hu TGFα in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TGFα. Principle of the method The Human TGFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Transforming growth factor (TGF) alpha is a 50-aa single-chain polypeptide derived from cleavage of transmembrane pro-TGF alpha. Sharing 42% identity with epidermal growth factor (EGF), TGF alpha binds the EGF receptor and activates downstream signaling pathways. TGF alpha is expressed by activated macrophages, keratinocytes, epithelial cells, and a variety of tumor-derived and transformed cell lines. This growth factor mediates cell proliferation and migration, as well as cellular transformation. Overexpression of TGF alpha has been linked to a variety of cancers, including the breast, colon, and stomach.
Specifications

Specifications

P01135
3 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
7039
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Angiogenesis
2°C to 8°C
Human
4 hr. 45 min.
1.37-1000 pg/mL
3pg/mL to 1000pg/mL
Biotin
Growth Factors & Receptors
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL- 13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).
Human
Colorimetric Microplate Reader
TGFA
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
TFGA
1 hr. 20 min.
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