Invitrogen™ TNFR1 (Soluble-60 kDa) Human ELISA Kit

For the quantitative detection of human sTNF-R (60 kd).

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal polyclonal antibody to human sTNF-R (60 kDa)
HRP-Conjugate anti-human sTNF-R (60 kDa) monoclonal antibody
Human sTNF-R (60 kDa) Standard, 10ng/mL upon dilution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Product Code	Brand	Quantity
15541527	Invitrogen™ BMS203	96 Tests
15551547	Invitrogen™ BMS203TEN	10 x 96 Tests

Description

The Human Soluble Tumor Necrosis Factor Receptor 1 (sTNFR1-60 kDa) ELISA quantitates sTNFR1 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sTNFR1. Principle of the method The Human sTNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

• Provides a simple, rapid and highly sensitive method for the determination of soluble TNF-R (60 kd) levels in body fluids or cell culture supernatants
• This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kd) in various neoplastic and inflammatory diseases

Specifications

• Accession Number: P19438
• Assay Sensitivity: 0.05 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60
• Intraassay CV: 1.9%
• Label or Dye: HRP
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL
• Synonym: Tumor necrosis factor receptor 1, TNF-R1, CD120a
• Test Time: 1 hr. 20 min.

• Assay Range: 0.08-5.0 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 7132
• Interassay CV: 8.6%
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, Detection Antibody Conjugate: anti-rabbit-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

For Research Use Only