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Invitrogen™ PSGL-1/CD162 (Soluble) Human ELISA Kit

For the quantitative detection of human PSGL-1.

Brand:  Invitrogen™ BMS255

300.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15521837

  • £451.00 / 96 tests
Estimated Shipment: 11-04-2024
to see stock.
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human PSGL-1
Biotin-Conjugate anti-human PSGL-1 monoclonal antibody
Streptavidin-HRP
Human PSGL-1 Standard lyophilized, 100 U/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human P-selectin Glycoprotein Ligand-1 (Hu PSGL-1/CD162) ELISA quantitates Hu PSGL-1/CD162 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PSGL-1/CD162. Principle of the method The Human PSGL-1/CD162 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
Specifications

Specifications

Q14242
1.0 U/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD162,CLA,PSGL-1,PSGL1
3.2%
HRP
RUO
2°C to 8°C
Human
3 hr. 10 min.
1.56-50 U/mL
Biotin
Serum, Supernatant
ELISA
6404
6.6%
Pre-coated 96 well plate, Standard, Sample Diluent, Sample Diluent, Assay Buffer concentrate, SAV-HRP, Wash Buffer, Wash Buffer, Chromogen, Stop Solution, Controls
96 Tests
Serum, 15 μL; Supernatant, 100 μL
P-selectin glycoprotein ligand 1, CD162 antigen
1 hr. 20 min.
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