Invitrogen™ CD31/PECAM (Soluble) Human ELISA Kit

For the quantitative detection of human sPECAM-1.

Brand:  Invitrogen™ BMS229TEN

Product Code. 15501777

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sPECAM-1
HRP-Conjugate anti-human sPECAM-1 monoclonal antibody
Human sPECAM-1 Standard, 60ng/mL
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

The Human Soluble CD31/Platelet Endothelial Cell Adhesion Molecule(Hu sCD31/PECAM) ELISA quantitates Hu sCD31/PECAM in human serum, plasma, cell culture supernatants, urine, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD31/PECAM. Principle of the method The Human sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. The N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. Further, CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. CD31 is one of key regulatory molecules in the vascular system. CD31 induces susceptibility to atherosclerosis and this cell adhesion molecule is required for leukocyte migration under most inflammatory conditions. Antibodies to CD31 is of value in the study of benign and malignant vascular tumors.

Specifications

• Accession Number: P16284
• Assay Sensitivity: 0.06 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD31,CD31/EndoCAM,GPIIA′,PECA1,PECAM-1,endoCAM
• Intraassay CV: 1.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 0.47-30.0 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 5175
• Interassay CV: 7.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL
• Synonym: Leukocyte surface protein, CD31, EndoCAM, HEC7
• Test Time: 1 hr. 20 min.

For Research Use Only