Invitrogen™ L-Selectin (CD62L) (Soluble) Human ELISA Kit

For the quantitative detection of human sL-selectin.

Brand:  Invitrogen™ BMS206TEN

Product Code. 15551587

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sL-selectin
HRP-Conjugate anti-human sL-selectin monoclonal antibody, ready to use
Human sL-selectin Standard lyophilized, 50ng/mL upon reconstitution
Sample Diluent
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

The Human L-Selectin (CD62L) ELISA quantitates Hu L-Selectin in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu L-Selectin. Principle of the method The Human L-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD62L (L-selectin) is an adhesion glycoprotein with a molecular weight range of 74-95 kDa that is constitutively expressed on the cell surface of leukocytes, and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similar to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). Single-nucleotide polymorphisms in the CD26L gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for CD26L.

Specifications

• Accession Number: P14151
• Assay Sensitivity: 0.198 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD62L,LAM1,LECAM1,LEU8,LNHR,LSEL,LYAM1,PLNHR,TQ1
• Intraassay CV: 3.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 10 min.

• Assay Range: 0.4-25.0 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 6402
• Interassay CV: 4.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Synonym: Lymphocyte adhesion molecule 1, CD62L, Leu-8
• Test Time: 1 hr. 20 min.

For Research Use Only