Invitrogen™ IL-6 Receptor (Soluble) Human Instant ELISA™ Kit

Instant ELISA Kit

Brand:  Invitrogen™ BMS214INST

Product Code. 15561687

Includes: Aluminum pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sIL-6R and HRP-Conjugate (anti-sIL-6R monoclonal (murine) antibody), lyophilized
Aluminum pouch(es) with a human sIL-6R Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Stop Solution (1M Phosphoric acid)
Lyophilized Control
Adhesive Plate Covers

Description

The Human Soluble Interleukin 6 Receptor(Hu sIL-6R) ELISA quantitates Hu sIL-6R in human serum, plasma, cell culture supernatants, urine, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIL-6R. Principle of the method The Human sICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL6R (interleukin-6 receptor subunit alpha) is a part of the receptor for interleukin 6. The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.

Specifications

• Accession Number: P08887
• Assay Sensitivity: 0.01 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD126,IL-6R-1,IL-6RA,IL6QA,IL6RQ,gp80,IL6R
• Intraassay CV: 6.8%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: -20°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 78-5,000 pg/mL
• Conjugate: HRP
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 3570
• Interassay CV: 8.4%
• Kit Contents: Pre-coated 96 well Microplate, Adhesive Plate Covers, Detection Antibody, Sample Diluent, lyophilized, Standard curve strips, Standard curve strips, Substrate Solution, Substrate Solution, Stop Solution, Lysis Buffer 10x
• Quantity: 128 Tests
• Sample Volume: Amniotic Fluid, 10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL
• Synonym: CD126, Membrane glycoprotein 80
• Test Time: 20 min.

For Research Use Only