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Invitrogen™ ICAM-3 (Soluble) Human ELISA Kit

For the quantitative detection of human sICAM-3.

Brand:  Invitrogen™ BMS218

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15551707

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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-3
HRP-Conjugate anti-human sICAM-3 monoclonal antibody
Human sICAM-3 Standard, 50ng/mL
Sample Diluent
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

The Human Intercellular Adhesion Molecule-3 (Hu ICAM-3) ELISA quantitates Hu ICAM-3 in human serum, plasma, amniotic fluid, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ICAM-3. Principle of the method The Human ICAM-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Specifications

Specifications

P32942
0.38 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD50,CDW50,ICAM-R
2.5%
HRP
RUO
2°C to 8°C
Human
2 hr. 10 min.
0.78-50 ng/mL
HRP
Amniotic Fluid, Plasma, Serum, Supernatant, Urine
ELISA
3385
2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Amniotic Fluid, 20 μL; Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL; Urine,20 μL
CD50 antigen, Intercellular adhesion molecule 3
1 hr. 20 min.
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