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Invitrogen™ CD40L (Soluble) Human ELISA Kit, Extra Sensitive

For the quantitative detection of human sCD40L.

£451.00 - £3070.00

Specifications

Accession Number P29965
Assay Range 0.08-5.0 ng/mL
Assay Sensitivity 0.01 ng/mL
Conjugate HRP
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
HRP-Conjugate anti-human sCD40L monoclonal antibody
Human sCD40L Standard lyophilized, 20ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15561897
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Invitrogen™
BMS293TEN
10 x 96 Tests
£3070.00
Pack of 10
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15541897
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Invitrogen™
BMS293
96 Tests
£451.00
96 tests
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Estimated Shipment: 23-04-2024
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Description

Description

The Human CD40 Ligand (CD40L) ELISA quantitates Hu CD40L in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD40L. Principle of the method The Human CD40L US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P29965
0.01 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L
5.5%
HRP
Plasma, 50 μL; Serum, 20 μL; Supernatant, 50 μL
TRAP, HIGM 1, T cell antigen Gp39, CD154
1 hr. 20 min.
0.08-5.0 ng/mL
HRP
Plasma, Serum, Supernatant
ELISA
959
6.6%
Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Human
3 hr. 10 min.
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