Invitrogen™ CD23 (Soluble) Human ELISA Kit

For the quantitative detection of human sCD23.

Brand:  Invitrogen™ BMS227-2TEN

Product Code. 15511767

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD23
Biotin-Conjugate anti-human sCD23 monoclonal antibody
Streptavidin-HRP
Human sCD23 Standard lyophilized, 400 U/ml upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Human Soluble CD23 (Hu sCD23) ELISA quantitates Hu sCD23 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sCD23. Principle of the method The Human sCD23 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

Specifications

• Accession Number: P06734
• Assay Sensitivity: 6.8 U/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: BLAST-2,CD23,CD23A,CLEC4J,FCE2,IGEBF
• Intraassay CV: 4%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 12.5-400 U/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 2208
• Interassay CV: 6.3%
• Kit Contents: Pre-coated 96 well plate, Adhesive Plate Covers, Sample Diluent, Sample Diluent, Biotinylated Detection Antibody, Biotinylated Detection Antibody, Wash Buffer, Wash Buffer, Chromogen, Controls, Controls
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Synonym: Immunoglobulin E receptor low affinity II
• Test Time: 1 hr. 20 min.

For Research Use Only