Invitrogen™ CD137 (4-1BB) (Soluble) Human ELISA Kit

For the quantitative detection of human sCD137.

Brand:  Invitrogen™ BMS289TEN

Product Code. 15501897

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD137
Biotin-Conjugate anti-human sCD137 polyclonal antibody
Streptavidin-HRP
Human sCD137 Standard lyophilized, 100ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Human Soluble CD137 (4-1BB) (Hu CD137) ELISA quantitates Hu CD137 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD137. Principle of the method The Human CD137 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD137 is a member of the TNF-receptor superfamily and contributes to the clonal expansion, survival, and development of T cells. CD137 exists as both a monomer and a dimer on the surface of activated T cells. CD137 can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of CD137 is induced by lymphocyte activation. CD137 is also an inducible receptor-like protein expressed on the cell surface of activated splenic T cells and thymocytes. CD137 exists as both a monomer and a dimer on the surface of activated T cells.

Specifications

• Accession Number: Q07011
• Assay Sensitivity: 0.2 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: 4-1BB,CD137,CDw137,ILA
• Intraassay CV: 7.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 0.78-50 ng/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3604
• Interassay CV: 9.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: 4-1BB Ligand Receptor, TNFRSF9, ILA
• Test Time: 1 hr. 20 min.

For Research Use Only