Invitrogen™ sCD134/OX40 (Soluble) Human ELISA Kit

For the quantitative detection of human sCD134/OX40.

Brand:  Invitrogen™ BMS296TEN

Product Code. 15358120

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD134/OX40
Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
Streptavidin-HRP
Human sCD134/OX40 Standard lyophilized, 10ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Human soluble CD134/OX40 ELISA quantitates Hu CD134/OX40 in human serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD134/OX40. Principle of the method The Human CD134/OX40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

OX40 (CD134) is a member of the TNF-receptor superfamily, and has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that OX40 promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.OX40 is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary costimulatory molecule, expressed after 24 to 72 hours following activation. OX40L, a ligand of OX40, is also not expressed on resting antigen presenting cells. Expression of OX40 is dependent on full activation of the T cell; without CD28, expression of OX40 is delayed and expressed at lower levels.

Specifications

• Accession Number: P43489
• Assay Sensitivity: 1.8 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ACT35,CD134,IMD16,OX40,TXGP1L
• Intraassay CV: 8%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 78-5,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 7293
• Interassay CV: 12%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard, Sample Diluent, Biotinylated Detection Antibody, SAV-HRP, SAV-HRP, Lysis Buffer, Lysis Buffer, Stop Solution, Stop Solution
• Quantity: 10 x 96 Tests
• Sample Volume: Serum, 25 μL; Supernatant, 25 μL
• Synonym: OX 40L receptor, ACT 35
• Test Time: 1 hr. 20 min.

For Research Use Only