Thermo Scientific™ Pierce™ Human RANTES ELISA Kits

Measure levels of regulated upon activation, normal T-cell expressed and secreted (RANTES) in human serum, plasma, tissue extracts, urine and culture supernatant samples.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EHRNTS5

HUMAN RANTES ELISA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10763115

Quantity Price
1 £ 1568.0 / 1 set
Estimated Shipment date
from Supplier 02-02-2017
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Description and Specification

Specification

Precision Intra-assay CV <10%; inter-assay CV <10%
Sensitivity <2pg/mL
Specificity No significant cross-reaction with human Eotaxin, GROa, GROß, MIP-1a, MIP-1ß, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNa, IFNg, IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFa, TNFß, or rat RANTES.
Formulation Complete ELISA kit with coated 96-well plates
Measurement Absorbance at 450nm minus absorbance at 550nm
Kit Contents 30X Wash Buffer, 200mL
Assay Range 51.2 to 2000pg/mL
Sufficient For 5 x 96 microplate wells
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Description Human RANTES ELISA Kit
Sample Size 50µL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Target Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)
Total Assay Time 3 hr.
Quantity 5 plate kit

These human RANTES ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES (regulated upon activation, normal T-cell expressed and secreted) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 51.2 to 2000pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

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