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Invitrogen™ eBioscience™ Human PrimeFlow™ RNA Assay

In situ hybridization assay that combines the power of the ViewRNA™ branched DNA technology with the single cell resolution of flow cytometry. Branched DNA technology uses pairs of target-specific probes to achieve 8,000 to 16,000 fold signal amplification from a desired mRNA transcript.

Brand:  Invitrogen™ 88-18009-204

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15530857

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Contains Sodium azide and <10% formaldehyde

Includes: PrimeFlow™ RNA Tubes
PrimeFlow™ RNA Fixation Buffer 1A
PrimeFlow™ RNA Fixation Buffer 1B
PrimeFlow™ RNA Permeabilization Buffer (10X)
PrimeFlow™ RNA Fixation Buffer 2 (8X)
PrimeFlow™ RNA Wash Buffer
PrimeFlow™ RNA Target Probe Diluent
PrimeFlow™ RNA PreAmp Mix
PrimeFlow™ RNA Amp Mix
PrimeFlow™ RNA Label Probe Diluent
PrimeFlow™ RNA Storage Buffer
Rnase Inhibitor 2 (100X)
PrimeFlow™ RNA Label Probes
Rnase Inhibitor 1 (1000X)
Positive Control Probe Sets:
Type 1 Human RPL13A Alexa Fluor 647
Type 4 Human RPL13A Alexa Fluor 488
Type 6 Human RPL13A Alexa Fluor 750



  • Simultaneous detection of up to three RNA targets
  • Combine with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations
  • This Human PrimeFlow™ RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423)
  • Target Probe sets for genes of interest are sold separately
  • For the study of mouse samples, use the Mouse PrimeFlow™ RNA Assay (cat. 88-18001)
  • For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.


Flow Cytometric Analysis
40 tests
Storage conditions vary, see components list for details

For Research Use Only