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eBioscience™ Human PrimeFlow™ RNA Assay

In situ hybridization assay that combines the power of the ViewRNA™ branched DNA technology with the single cell resolution of flow cytometry. Branched DNA technology uses pairs of target-specific probes to achieve 8,000 to 16,000 fold signal amplification from a desired mRNA transcript.

Brand:  Affymetrix eBioscience 88-18009-204

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15530857

  • £1028.03 / 40 tests


Estimated Shipment date
from Supplier 25-09-2017
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Alert:

Contains Sodium azide and <10% formaldehyde

Disclaimers:

For Research Use Only


Includes: PrimeFlow™ RNA Tubes
PrimeFlow™ RNA Fixation Buffer 1A
PrimeFlow™ RNA Fixation Buffer 1B
PrimeFlow™ RNA Permeabilization Buffer (10X)
PrimeFlow™ RNA Fixation Buffer 2 (8X)
PrimeFlow™ RNA Wash Buffer
PrimeFlow™ RNA Target Probe Diluent
PrimeFlow™ RNA PreAmp Mix
PrimeFlow™ RNA Amp Mix
PrimeFlow™ RNA Label Probe Diluent
PrimeFlow™ RNA Storage Buffer
Rnase Inhibitor 2 (100X)
PrimeFlow™ RNA Label Probes
Rnase Inhibitor 1 (1000X)
Positive Control Probe Sets:
Type 1 Human RPL13A Alexa Fluor 647
Type 4 Human RPL13A Alexa Fluor 488
Type 6 Human RPL13A Alexa Fluor 750

Description

Description

  • Simultaneous detection of up to three RNA targets
  • Combine with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations
  • This Human PrimeFlow™ RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for human ribosomal protein, RPL13A (NM_012423)
  • Target Probe sets for genes of interest are sold separately
  • For the study of mouse samples, use the Mouse PrimeFlow™ RNA Assay (cat. 88-18001)
  • For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.
Specifications

Specifications

Flow Cytometric Analysis
40 tests
Human
Storage conditions vary, see components list for details