Learn More
Invitrogen™ PGF Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHPGF
362.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Code : NEW
Additional Details : Weight : 0.00400kg
Description
The Human Placental Growth Factor (PLGF) (Hu PGF) ELISA quantitates Hu PGF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGF. Principle of the method The Human PGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor affects different cell types and regulates various biological responses. The activity of vascular endothelial growth factor (VEGF), which is the most potent of all angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in human placenta.Specifications
P49763 | |
2 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
5228 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Angiogenesis | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
1.372-1000 pg/mL | |
2pg/mL to 1000pg/mL | |
Biotin | |
Growth Factors & Receptors | |
This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
Human | |
Colorimetric Microplate Reader | |
D12S1900L,PIGF,PLGF,PlGF-2,SHGC-10760,PGF | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
D12S1900L, PLGF, PlGF-2, SHGC-10760, PGF | |
1 hr. 20 min. |