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eBioscience™ Human PEI-Transferrinfection

Nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells and the gene transfer efficiency of PEI/DNA complexes

Brand:  Affymetrix eBioscience BMS1003

Code : NEW

Additional Details : Weight : 0.47500kg

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Product Code. 15531307

  • £180.17 / 32 reactions


Estimated Shipment date
from Supplier 30-10-2017
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Includes: Transferrin-Polyethylenimine-conjugate, lyophilized, 1 mg/mL upon reconstitution; iron incorporated
Deferrioxamine, lyophilied, 50 mM upon reconstitution
HBS buffer concentrate (20x)
(400mM Hepes, pH 7.3, 3M NaCl)

Description

Description

  • Method results in a 30 to 1000-fold enhanced transfection efficiency depending on the cell line
  • An extremely gentle DNA transfection method which employs physiological uptake mechanisms of the cell
  • Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression
  • Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines
  • In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure
Specifications

Specifications

Cell Culture
32 transferrinfections
Human