Invitrogen™ PD-L2 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ BMS2215

Product Code. 15581727

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human PD-L2

Description

The Human Programmed Death-1 Receptor Ligand (Hu PD-L2) ELISA quantitates Hu PD-L2 in human serum, plasma,cell lysates, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PD-L2. Principle of the method The Human PD-L2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD273 (PDCD1 Ligand 2, Programmed cell death 2 ligand 2, PD-L2, B7-DC) is involved in cell-mediated immune responses initiated by T lymphocytes that are stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as CD273 that mediate inhibitory signals from the APC (antigen presenting cell). CD273 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; CD273 is specific to the largest isoform. Diseases associated with CD273 include cysticercosis, and myasthenic syndrome.

Specifications

• Accession Number: Q9BQ51
• Assay Sensitivity: 3.65 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PDCD1LG2
• Intraassay CV: 4.5%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 39-2,500 pg/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 80380
• Interassay CV: 3.1%
• Kit Contents: Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Assay Buffer concentrate, Assay Buffer concentrate, Biotinylated Detection Antibody, Wash Buffer, Chromogen, Chromogen, Stop Solution, Controls
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Synonym: PDCD1LG2, B7DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2, bA574F11.2
• Test Time: 1 hr. 20 min.