Invitrogen™ MMP10 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHMMP10

Product Code. 15435073

Description

The Human Matrix MetalloProteinase 10 (Hu MMP10) ELISA quantitates Hu MMP10 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP10. Principle of the method The Human MMP10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MMP10 belongs to the matrix metalloproteinase family, and is generally secreted in its pro form, which is further modified by extracellular proteinases. As a metalloproteinase, MMP10 requires zinc and calcium as cofactors for activity. It is involved in the activation of pro-collagenase, catabolism of collagen, degradation of fibronectin, and tissue remodeling. In humans, the gene MMP10 located on chromosome 11.

Specifications

• Accession Number: P09238
• Assay Sensitivity: 3 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 4319
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 2.06-1500 pg/mL
• Calibration Range: 3pg/mL to 1500pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, EGF, ENA-78, FGF-4, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, 6, 7, 9, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -9,- 12,-13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: SL-2,STMY2
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: SL-2, STMY2
• Test Time: 1 hr. 20 min.