missing translation for 'onlineSavingsMsg'
Learn More

Human MIP-1 alpha ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of human MIP-1 alpha (MIP-1a)

Brand:  Thermo Scientific Pierce Ab EH2MIP1A5

Code : NEW

Additional Details : Weight : 0.01000kg

Product Code. 15328244

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance
View alternative products »


Includes: Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description

Description

These human MIP-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of macrophage inflammatory protein-1 alpha (MIP-1α) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human MIP-1α (macrophage inflammatory protein-1 alpha)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 2.5pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with human MIP-1β, MIP-1δ, MIP-1α, MIP-3β; mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β; or rat MIP-3α, MIP-I and MIP-II

Specifications

Specifications

calibrated recombinant protein standard
streptavidin-HRP
TMB substrate solution and stop solution
pre-coated 96-well strip plate(s)
lot-optimized reagents
5 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human MIP-1β, MIP-1δ, MIP-3α, MIP-3β mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β or rat MIP-3β, MIP-I and MIP-II.
3 hours
Intra-assay CV < 10%; inter-assay CV < 10%
< 2.5pg/mL
50μL
5 x 96 microplate wells
Natural and recombinant human MIP-1 α(macrophage inflammatory protein-1 alpha)
Videos