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Invitrogen™ Leptin Human Instant ELISA™ Kit
Instant ELISA Kit
Brand: Invitrogen™ BMS2039INST
Code : NEW
Additional Details : Weight : 0.23750kg
Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Leptin, Biotin-Conjugate (anti-human Leptin monoclonal antibody), Streptavidin-HRP and Assay Buffer, lyophilized
Aluminum pouch(es) with a human Leptin Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Description
The Human Leptin) ELISA quantitates Hu Leptinin human serum, plasma or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Leptin. Principle of the method The Human Leptin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.Specifications
| P41159 | |
| 20.0 pg/mL | |
| ELISA Kit | |
| Human | |
| Colorimetric Microplate Reader | |
| LEPD,OB,OBS | |
| 5.7% | |
| HRP | |
| RUO | |
| -20°C | |
| Human | |
| 3 hr. 10 min. |
| 63-4,000 pg/mL | |
| Biotin | |
| Plasma, Serum | |
| ELISA | |
| 3952 | |
| 6.9% | |
| Pre-coated 96 well Microplate, Adhesive Plate Covers, Biotinylated Detection Antibody, Sample Diluent, lyophilized, lyophilized, Standard curve strips, Substrate Solution, Stop Solution, Stop Solution, Lysis Buffer 10x | |
| 128 Tests | |
| Plasma, 10 μL; Serum, 10 μL | |
| Obesity factor, Obese protein | |
| 20 min. |
For Research Use Only