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Thermo Scientific™ anti-Interleukin 8 Recombinant, Thermo Scientific™

Interleukin 8 Recombinant protein SIL8 from Thermo Scientific™ specifically detects Interleukin 8 in Not Applicable samples, and it is validated for Control, ELISA and WB

Brand:  Thermo Scientific™ EH2IL82

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10025653

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These human IL-8 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-8 (IL-8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-8 antibody pairs and IL-8 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 1000pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with human PF-4, GROα, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFα, IFNα, IFNγ, or GM-CSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

25.6 to 1000pg/mL
Human IL-8 ELISA Kit
Complete ELISA kit with coated 96-well plates
Absorbance at 450nm minus absorbance at 550nm
<2pg/mL
50µL
2 x 96 microplate wells
Natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
Adhesive Plate Covers, 12 ea
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
2 plate kit
Culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with with human PF-4, GROa, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFa, IFNa, IFNg, or GM-CSF
3 hr.