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Invitrogen™ eBioscience™ Mouse HMGB1, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Brand:  Invitrogen™ 34-8401-85

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15508806

  • £1988.29 / 500µg

Estimated Shipment date
from Supplier 03-07-2018
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High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4.


Cytokine Bioassays
The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6kDa.
Store at less than or equal to -70°C.
E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439
high mobility group box 1
Recombinant Protein, Carrier-Free
Sterile liquid; 20mM Tris-HCl, pH 8.0, 0.2M NaCl, 1mM DTT. 0.22μm filtered.
E. coli
Less than 0.01ng/μg cytokine as determined by the LAL assay.
The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25μg/mL in the presence of 10μg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.