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Invitrogen™ IL-6 Human ELISA Kit, High Sensitivity

High Sensitivity Sandwich ELISA Kit

Brand:  Invitrogen™ BMS213HS

Code : NEW

Additional Details : Weight : 0.23750kg

Product Code. 15521687

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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
Biotin-Conjugate anti-human IL-6 monoclonal antibody
Streptavidin-HRP
Human IL-6 Standard lyophilized, 200pg/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent
Amplification Diluent Concentrate (2x)
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human High Sensitivity Interleukin 6 (HS IL-6) ELISA quantitates Hu HS IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-6. Principle of the method The Human HS IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Specifications

Specifications

P05231
0.03 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
4.9%
HRP
RUO
2°C to 8°C
Human
4 hr.
0.08-5.0 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3569
6%
Pre-coated 96 well plate, Standard, Chromogen, Assay Buffer concentrate, Adhesive Plate Covers, Standard, Amplification Reagent I, Assay Buffer concentrate, SAV-HRP, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Controls
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Interferon-beta 2, B-cell stimulatory factor 2,HGF
1 hr. 20 min.
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