eBioscience™ Human GM-CSF, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8339-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Product Code. 15578786

Quantity Price
1 £ 470.18 / 100µg
EU Stock 5
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Description and Specification


Regulatory Status RUO
Conjugate Unlabeled
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl. 0.22μm filtered.
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 14,478. The DTT reduced protein migrates as a 14kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 13kDa polypeptide.
Recombinant Yes
Gene Symbol Granulocyte/Macrophage-Colony Stimulating Factor
Storage Requirements Store at less than or equal to -70°C.
Product Type Recombinant Protein, Carrier-Free
For Use With (Application) Cytokine Bioassays
Purification Method SDS-PAGE
Quantity 100μg
Structural Form E. coli expressed amino acids ala 18 - glu144 of mature human GMCSF accession # NM_000758
Accession Number NM_000758
Purity ≥95%
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Source E. coli
Biological Activity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 100pg/mL. This corresponds to a specific activity of greater than or equal to 1 x 107 Units/mg.
Cross Reactivity Human

Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF alpha, IL-1, IL-2 and IFN gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.