eBioscience™ Mouse IL-33, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8332-85

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15568786

Quantity Price
1 £ 1570.72 / 500µg
EU Stock 1
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Description and Specification

Specification

Purification Method SDS-PAGE
Regulatory Status RUO
Molecular Weight The DTT reduced protein migrates as a 15kDa polypeptide on SDS-PAGE.
Quantity 500μg
Cross Reactivity Mouse
Product Type Recombinant Protein, Carrier-Free
Storage Requirements Store at less than or equal to -70°C.
Structural Form E. coli expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33 accession # AK075849
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Biological Activity Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.03ng/mL, corresponding to a specific activity of 3.0 x 107 Units/mg.
For Use With (Application) Cytokine Bioassays
Name IL-33
Formulation Sterile liquid; 10mM phosphate, 150mM NaCl, 2mM TCEP, pH 7.2. 0.22μm filtered.
Source E. coli
Recombinant Yes
Purity >97%
Gene Symbol Interleukin-33
Accession Number AK075849
Conjugate Unlabeled

Mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of alarmins also known as endogeneous danger signals. Other known alarmins are IL-1 alpha; and HMGB1. IL-33 has been identified as the ligand for ST2 (a member of the Il-1 receptor family). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat.