eBioscience™ Mouse IL-33, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 34-8332-85

500UG Mouse IL-33 Recombinant Protein Carrier-Free

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15568786

Quantity Price
1 £ 2393.5 / 500µg
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

Accession Number AK075849
Conjugate Unlabeled
Cross Reactivity Mouse
For Use With (Application) Cytokine Bioassays
Formulation Sterile liquid; 10mM phosphate, 150mM NaCl, 2mM TCEP, pH 7.2. 0.22μm filtered.
Molecular Weight The DTT reduced protein migrates as a 15kDa polypeptide on SDS-PAGE.
Name IL-33
Purification Method SDS-PAGE
Purity >97%
Quantity 500μg
Recombinant Yes
Source E. coli
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Structural Form E. coli expressed protein consisting of amino acids Ser 109-Ile 266 of mature mouse IL-33 accession # AK075849
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Gene Symbol Interleukin-33
Biological Activity Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.03ng/mL, corresponding to a specific activity of 3.0 x 107 Units/mg.
Product Type Recombinant Protein, Carrier-Free

Mouse IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by endothelial and epithelial cells. IL-33 is released during necrotic cell death but was first found in the nucleus of endothelial cells. This dual pattern of expression is reminiscent of alarmins also known as endogeneous danger signals. Other known alarmins are IL-1 alpha; and HMGB1. IL-33 has been identified as the ligand for ST2 (a member of the Il-1 receptor family). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-mediated inflammation. Although IL-33 was initially reported to be processed by Caspase-1, recent data indicate that Caspase-3 or -7 are capable of processing IL-33 at more physiologic concentrations. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13 in vitro and in vivo and activating basophils, eosinophils and mast cells. In addition to its role in proimflammation, it may also decrease inflammation through interactions with IL-1 thereby blocking its effect. Mouse IL-33 share 55% amino acid sequence identity with human and 90% with rat.