Invitrogen™ IL-4 Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of human IL-4 protein levels from samples including serum, plasma, and cell culture supernatants.

Brand:  Invitrogen™ 88-7046-22

Product Code. 15541017

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P05112
• Product Type: ELISA Kit
• Assay Range: 2-200 pg/mL
• Gene ID (Entrez): 3565
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 24 hr. 30 min.
• Synonym: Interleukin-4
• Target Species: Human
• Assay Sensitivity: 2 pg/mL
• For Use With (Equipment): Colorimetric Microplate Reader

• Label or Dye: HRP
• Conjugate: Biotin
• For Use With (Application): ELISA
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Streptavidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
• Sample Volume: Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
• Test Time: 1 hr. 45 min.
• Target: Human
• Quantity: 2 x 96 Tests
• Sample Type: Supernatant
• Gene Symbol: IL-27,IL-27AA,IL27p28,IL30,p28,IL27

For Research Use Only