Human IL-4 ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of human IL-4 (IL-4)

Overview
Brand: Invitrogen

Manufacturer Part Number: EH3IL45

Human IL-4 ELISA Kit, 5x 96 well plate kit

UNSPSC: 12352203

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15368224

Quantity Price
1 £ 1330.0 / Pack of 5
Estimated Shipment date
from Supplier 19-12-2016
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Description and Specification

Specification

Formulation Complete ELISA kit with coated 96-well plates
Includes Anti-Human IL-4 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Human IL-4 Standards, 10 vials
Standard Diluent, 5 x 26mL
30X Wash Buffer, 5 x 50mL
Anti-Human IL-4-HRP Concentrate, 5 x 75μL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 55mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 20 each
Incubation Time 3.5 hours
Precision intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Quantity 5-plate kit
Sensitivity ≤ 2pg/mL
Sample Type Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
Sample Size 50μL
Specificity No significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4
Sufficient For 5 x 96 microplate wells
Test Time 3.5 hours
Target Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)

The human IL-4 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1, BSF1) or B-cell growth factor 1 (BCGF-1, BCGF1), in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant human interleukin-4 (IL-4; also called BSF-1 or BCGF-1)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 10.24 to 400pg/mL
  • Sensitivity: ≤ 2pg/mL
  • Precision: Intra assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: Cell culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with human IL-2, IL-6, IL-10, IFNγ, GM-CSF or mouse IL-4