eBioscience™ Human Erythropoietin (EPO), Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8992-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15577556

Quantity Price
1 £ 286.76 / 25µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Accession Number P01588
Molecular Weight The predicted molecular mass of this protein is 20.6kDa. It migrates at 34kDa by SDS-PAGE due to glycosylation.
For Use With (Application) Cytokine Bioassays
Storage Requirements For greatest stability, keep concentration of primary stock at or above 10μg/mL. For long term storage, aliquot into polypropylene vials (volumes of 20μL or greater) and store at less than or equal to -70°C. Avoid repeated freeze/thaw cycles.
Biological Activity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is 0.8-1ng/mL. This corresponds to a specific activity of 1-1.25x 106 Units/mg.
Cross Reactivity Human
Conjugate Unlabeled
Recombinant Yes
Purity >96%
Source CHO Cells
Product Type Recombinant Protein
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay.
Name Erythropoietin (EPO)
Concentration 0.1mg/mL
Purification Method SDS-PAGE
Formulation Sterile liquid; phosphate buffered saline with 1% BSA, pH 7.2 0.22μm filtered.
Regulatory Status RUO
Quantity 25μg
Structural Form CHO expressed amino acids Ala28-Arg193 accession number P01588

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.

Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.