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Human Erythropoietin (EPO), Invitrogen™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8992-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15577556

  • £304.00 / 25µg

Estimated Shipment date
from Supplier 30-03-2020

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Description

Description

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair. Description: Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation. Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair. Applications Reported: Human EPO Recombinant Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is 0.8-1ng/mL. This corresponds to a specific activity of 1-1.25x 10e6 Units/mg. Source: CHO expressed amino acids Ala28-Arg193 accession number P01588. Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is 0.8-1ng/mL. This corresponds to a specific activity of 1-1.25x 10e6 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >96% as determined by SDS-PAGE. Molecular Weight: The predicted molecular mass of this protein is 20.6 kDa. It migrates at 34 kDa by SDS-PAGE due to glycosylation. Storage and handling: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent. For greatest stability, keep concentration of primary stock at or above 10ug/mL. For long term storage, aliquot into polypropylene vials (volumes of 20uL or greater) and store at less than or equal to -70°C. Avoid repeated freeze/thaw cycles. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

P01588
Control, Functional Assay
PBS with 1% BSA and no preservative; pH 7.2
The predicted molecular mass of this protein is 20.6kDa. It migrates at 34kDa by SDS-PAGE due to glycosylation.
7.2
Affinity chromatography
CHO Cells
RUO
Recombinant
Human Erythropoietin (EPO)
Control, Functional Assay
Recombinant Protein
Unlabeled
CHO cells
0.1mg/mL
Liquid
2056
Human Erythropoietin (EPO)
SDS-PAGE
25μg
-80°C
CHO expressed amino acids Ala28-Arg193 accession number P01588
Less than 0.1ng/μg cytokine as determined by the LAL assay.
EPO
The ED50 of this protein, as measured by TF-1 cell proliferation assay, is 0.8-1ng/mL. This corresponds to a specific activity of 1-1.25x 106 Units/mg.
Human
Hu
Recombinant
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