eBioscience™ Human IL-21, Recombinant Protein

Human Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8219-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg

Disclaimers: For Research Use Only.

Product Code. 15132139

Quantity Price
1 £ 243.96 / 25µg
EU Stock 2
Add to basket

Description and Specification


For Use With (Application) Cytokine Bioassays, ELISA Recombinant Standard
Molecular Weight The protein has a predicted molecular mass of 17,150. The DTT reduced protein migrates as a 18kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 17kDa polypeptide.
Regulatory Status RUO
Endotoxin Concentration Less than 0.01ng/ug cytokine as determined by the LAL assay.
Structural Form A DNA sequence encoding the mature form of human IL-21amino acids Gln25-Ser155 accession # NM_021803 was expressed in E.coli
Purification Method SDS-PAGE
Formulation Sterile liquid; 20mM sodium phosphate, pH 7.2, 0.6M NaCl, 1.0% BSA. 0.22μm filtered.
Quantity 25μg
Gene Symbol Interleukin-21, IL21
Purity >98%
Recombinant Yes
Conjugate Unlabeled
Source E. coli
Biological Activity The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 25ng/mL. This corresponds to a specific activity of greater than or equal to 4 x 104 Units/mg.
Validated Application ELISA, FA
Name IL-21
Cross Reactivity Human
Accession Number NM_021803
Concentration 0.1mg/mL

Human Interleukin-21 (IL-21) is a 131-amino acid protein with 57% identity to the mouse gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma.