eBioscience™ Human IL-21, Recombinant Protein

Human Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 14-8219-62

5UG Human IL-21 Recombinant Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15517516

Quantity Price
1 £ 148.1 / 5µg
Estimated Shipment date
from Supplier 24-01-2017
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Description and Specification

Specification

Accession Number NM_021803
Concentration 0.1mg/mL
Conjugate Unlabeled
Cross Reactivity Human
For Use With (Application) Cytokine Bioassays , ELISA Recombinant Standard
Formulation Sterile liquid; 20mM sodium phosphate, pH 7.2, 0.6M NaCl, 1.0% BSA. 0.22μm filtered.
Molecular Weight The protein has a predicted molecular mass of 17,150. The DTT reduced protein migrates as a 18kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 17kDa polypeptide.
Name IL-21
Purification Method SDS-PAGE
Purity >98%
Quantity 5μg
Recombinant Yes
Source E. coli
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Structural Form A DNA sequence encoding the mature form of human IL-21amino acids Gln25-Ser155 accession # NM_021803 was expressed in E.coli
Endotoxin Concentration Less than 0.01ng/ug cytokine as determined by the LAL assay.
Gene Symbol Interleukin-21, IL21
Biological Activity The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 25ng/mL. This corresponds to a specific activity of greater than or equal to 4 x 104 Units/mg.
Product Type Recombinant Protein

Human Interleukin-21 (IL-21) is a 131-amino acid protein with 57% identity to the mouse gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma.