eBioscience™ Human Wnt-1, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 34-8695-61

2UG Human Wnt-1 Recombinant Protein Carrier-Free

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15598816

Quantity Price
1 £ 101.6 / 2µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Conjugate Unlabeled
Cross Reactivity Human
For Use With (Application) Functional Assays
Formulation Lyophilized without additive.
Molecular Weight 42kDa (mature form)
Name Wnt-1
Purification Method SDS-PAGE and HPLC
Purity >98%
Quantity 2μg
Recombinant Yes
Source E. coli
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Regulatory Status RUO
Structural Form E. coli (amino acids Ala28-Leu370; Accession # NP_005421)
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Biological Activity The ED50 of this protein, as determined by human BMP-2 (200ng/mL) induction of alkaline phosphatase production by murine ATDC5 cells, is 1.5-2.5ng/mL.
Product Type Recombinant Protein, Carrier-Free

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer.

Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.