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Invitrogen™ Human IL-2 ELISA Kits

Interleukin 2 Recombinant protein SIL2 from Thermo Scientific™ specifically detects Interleukin 2 in samples, and it is validated for ELISA and Western Blot

Brand:  Invitrogen™ ESS0010

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10454005

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Description

Description

These human IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-2 (IL-2)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 38.4 to 1500pg/mL

  • Sensitivity: < 6pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ, GM-CSF, INFγ, or mouse IL-2

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

38.4 to 1500pg/mL
Human IL-2 ELISA Reagent Kit
Antibodies and complete reagent set for ELISA
Absorbance at 450nm minus absorbance at 550nm
<6pg/mL
50µL
5 x 96 microplate wells
Natural and recombinant human interleukin-2 (IL-2)
Human IL-2 Standard, 5 vials
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
5 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, IL-1ß, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß, GM-CSF, INFg, or mouse IL-2
4 hr.
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