Invitrogen™ IL-17R Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHIL17R

Product Code. 15435033

Description

The Human Interleukin 17 Receptor (Hu IL-17) ELISA quantitates Hu IL-17 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-17. Principle of the method The Human IL-17 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.

Specifications

• Accession Number: Q96F46
• Assay Sensitivity: 10 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 23765
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 12.35-3000 pg/mL
• Calibration Range: 10pg/mL to 3000pg/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-17, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL17RA
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: CANDF5, CD217, CDw217, IL-17RA, IL17R, hIL-17R
• Test Time: 1 hr. 20 min.