Invitrogen™ IL-17F (Homodimer) Human Uncoated ELISA Kit

Specifically engineered for accurate and precise measurement of human IL-17F homodimer protein levels from samples including serum, plasma and cell culture supernatants.

Brand:  Invitrogen™ 88-7478-86

Product Code. 13121186

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X Carbonate ELISA Coating Buffer
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

The Human Interleukin 17F (Homodimer) (IL-17F) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17F. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human IL-17F ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)

• The assay demonstrates parallelism in measuring recombinant and native human IL-17F proteins and a standard curve range of 16 to 2000pg/mL
• Very specific to IL-17F homodimer. No crossreactivity to the IL-17A homodimer is observed and 0.24% crossreactivity to recombinant human IL-17AF heterodimer when spiked in excess (100ng/ml)

Specifications

• Assay Sensitivity: 16pg/mL
• For Use With (Application): ELISA
• Storage Requirements: 2°C to 8°C except standard which should be stored at ≤-70°C
• Target: IL-17F

• Target Species: Human
• Quantity: 10 x 96 Tests
• Synonym: Interleukin-17F, IL-17FF

For Research Use Only