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Invitrogen™ IL-17AF (heterodimer) Human Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of human IL-17AF protein levels from serum, plasma and cell culture supernatants. This assay demonstrates parallelism in measuring recombinant and native human IL-17AF proteins and a standard curve range of 30 to 4000pg/mL.
£174.00 - £391.00
Specifications
Accession Number | Q16552,Q96PD4 |
---|---|
Assay Range | 30-4,000 pg/mL |
Assay Sensitivity | 30 pg/mL |
Conjugate | Biotin |
Product Type | ELISA Kit |
Alert:
Contains Azide
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15571047
|
Invitrogen™
88-7117-22 |
2 x 96 Tests |
£174.00
Pack of 2 |
Estimated Shipment: 08-05-2024
Log in to see stock. |
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15581047
|
Invitrogen™
88-7117-86 |
10 x 96 Tests |
£391.00
Pack of 10 |
Estimated Shipment: 08-05-2024
Log in to see stock. |
|||||
Description
The Human IL-17AF (heterodimer) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17AF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Human IL-17AF ELISA Ready-SET-Go! Reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)
- Minimal crossreactivity to the IL-17A homodimer is observed (2%) and with no crossreactivity to IL-17F when spiked in excess (100ng/ml)
Specifications
Q16552,Q96PD4 | |
30 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
IL-5 | |
HRP | |
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL | |
Interleukin-IL-17AF, IL17AF | |
1 hr. 45 min. |
30-4,000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
112744, 3605 | |
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Assay Diluent: 5X concentrated, Wash Buffer: 10X concentrated (100 mL of 10X per plate), Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Stop Solution: 5 mLs of 1X solution per plate, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards | |
RUO | |
2°C to 8°C | |
Human | |
24 hr. 30 min. |
For Research Use Only