Invitrogen™ Human IL-1 alpha Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Invitrogen™ BMS328

Product Code. 15581927

Description

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Description: Interleukin-1 is a potent immuno-modulator, which mediates a wide range of immune and inflammatory responses. Human IL-1 is an 18.0 kDa protein containing 159 amino acid residues. The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct human IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation. IL-1alpha is an extracellular peptide of 17 kDa, its activity has been demonstrated in various biological fluids including serum, synovial fluid, gingival fluid, amniotic fluid, sputum, cerebrosinal fluid, urine, and bronchoalveolar lavage (BAL) fluid. Elevated serum or blood levels of IL-1alpha have been found in patients with total hip replacement/ arthroplasties, in patients with recently diagnosted IDDM (Insulin-Dependent Diabetes Mellitus), in case of several carcinomas such as head and neck cancer, pancreatic cancer and thyroid cancer, in experimental acute pyelonephritis, in acute viral hepatitis and in septic shock. Purity: >98%. Molecular Weight: 18.0 kDa.

Specifications

• Accession Number: P01583
• Formulation: TBS with no preservative; pH 8.0
• Molecular Weight (g/mol): 18.0kDa
• pH Range: 8.0
• Source: E. coli
• Regulatory Status: RUO
• Common Name: Human IL-1 alpha
• Biological Activity: We recommend a quick spin followed by reconstitution in water to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
• Specific Reactivity: Human
• Cross Reactivity: Hu
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• For Use With (Application): Control
• Gene ID (Entrez): 3552
• Name: Human IL-1 alpha
• Quantity: 10μg
• Storage Requirements: -20°C
• Structural Form: E. Coli
• Gene Symbol: IL1A
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: Affinity chromatography