Thermo Scientific™ Pierce™ Human IL-17A ELISA Kits

Assay human IL-17A levels in cell culture supernatants, plasma and serum samples.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: MA1-069B

X250 MONOCLONAL ANTIBODY, ANTI-IL17A , BIOTINConjugate (3-41.2.6.1), host mouse, clone 3-41.2.6

UNSPSC: 12352203

Code: LD

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 12847772

Quantity Price
1 £ 285.0 / 100µg
Estimated Shipment date
from Supplier 19-12-2016
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Description and Specification

Specification

Assay Range 31.25 to 2000pg/mL
Description Human IL-17A ELISA Reagent Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Antibodies and complete reagent set for ELISA
Kit Contents Human IL-17 Standard, 5 vials
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 5 plate kit
Sensitivity ≤ 5pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
Sufficient For 5 x 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant human interleukin-17A (also called CTLA8)

These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-17A (also called CTLA8)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.25 to 2000pg/mL

  • Sensitivity: ≤ 5pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.