Thermo Scientific™ Pierce™ Human IL-17A ELISA Kits
Assay human IL-17A levels in cell culture supernatants, plasma and serum samples.
Brand: Thermo Scientific™ EHIL17A
£333.00 valid until 31/05/2017
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UNSPSC : 41116126
Code : PN
Additional Details : Weight : 0.01000kg
DescriptionThese human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Target: Natural and recombinant human interleukin-17A (also called CTLA8)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 31.25 to 2000pg/mL
Sensitivity: ≤ 5pg/mL
Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3.5 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.
|31.25 to 2000pg/mL|
|Human IL-17A ELISA Kit|
|Complete ELISA kit with coated 96-well plate|
|Absorbance at 450nm minus absorbance at 550nm|
|96 microplate wells|
|Natural and recombinant human interleukin-17A (also called CTLA8)|
|30X Wash Buffer, 50mL|
|Colorimetric ELISA (TMB substrate) in clear 96-well strip plate|
|Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%|
|1 plate kit|
|Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)|
|No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF|
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