Thermo Scientific™ Pierce™ Human IL-17A ELISA Kits

Assay human IL-17A levels in cell culture supernatants, plasma and serum samples.

Overview
£333.00 valid until 31/03/2017
Log in to get the promotional price



Brand: Thermo Scientific™

Manufacturer Part Number: EHIL17A

HUMAN IL-17A COLORIMETRIC ELISA

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12371873

Quantity Price
1 £ 444.0 / Each
Estimated Shipment date
from Supplier 13-03-2017
Add to basket


Description and Specification

Specification

Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sensitivity ≤ 5pg/mL
Formulation Complete ELISA kit with coated 96-well plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Kit Contents 30X Wash Buffer, 50mL
Assay Range 31.25 to 2000pg/mL
Sufficient For 96 microplate wells
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Specificity No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Target Natural and recombinant human interleukin-17A (also called CTLA8)
Description Human IL-17A ELISA Kit
Total Assay Time 3.5 hr.
Quantity 1 plate kit

These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-17A (also called CTLA8)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.25 to 2000pg/mL

  • Sensitivity: ≤ 5pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Special Offers