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Invitrogen™ Human IL-17A ELISA Kits

Assay human IL-17A levels in cell culture supernatants, plasma and serum samples.

Brand:  Invitrogen™ EHIL17A

Code : PN

Additional Details : Weight : 0.01000kg

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Product Code. 12371873

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These human IL-17A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-17 (IL-17A, CTLA8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-17A (also called CTLA8)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.25 to 2000pg/mL

  • Sensitivity: ≤ 5pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Specifications

Specifications

31.25 to 2000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Human IL-17A Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 8mL, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-17A Standard, 2 vials, Sample Diluent, 14.5mL, Stop Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL
Absorbance at 450nm minus absorbance at 550nm
≤ 5pg/mL
50µL
96 microplate wells
Natural and recombinant human interleukin-17A (also called CTLA8)
Human IL-17A ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL17B, IL17D, IL17E, IL17F, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL16 or mouse IL17A, IL1A, IL1B or GMCSF
3.5 hr.
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