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Thermo Scientific™ Pierce™ Human IL-16 ELISA Kits

Measure levels of human interleukin-16 in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates. Thermo Scientific™ Interleukin 16 Recombinant protein SIL16 is validated for ELISA and Western Blot.

Brand:  Thermo Scientific™ EHIL16

Code : PN

Additional Details : Weight : 0.10000kg

Product Code. 10046953

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Description

Description

These human IL-16 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-16 (IL-16) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-16 (IL-16)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 0 to 2000pg/mL

  • Sensitivity: < 8pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.25 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human Eotaxin, G-CSF, GM-CSF, GROα, GROβ, IFNα, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-17, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, TNFα, TNFβ or RANTES

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

0 to 2000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL, Stop Solution, 13mL, Adhesive Plate Covers, 6 ea, Anti-Human IL-16 Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 8mL, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-16 Standard, 2 vials, Standard Diluent, 2 x 25mL, Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL
Absorbance at 450nm minus absorbance at 550nm
<8pg/mL
50µL
96 microplate wells
Natural and recombinant human interleukin-16 (IL-16)
Human IL-16 ELISA Kit
Complete ELISA kit with coated 96-well plate
Intra-assay CV <10%; inter-assay CV <10%
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human Eotaxin, G-CSF, GM-CSF, GROa, GROß, IFNa, IFNg, IL-1a, IL-1ß, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-17, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1a, MIP-1ß, TNFa, TNFß or RANTES.
3.25 hr.
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