Invitrogen™ IL-15 Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of protein levels from samples including serum, plasma, and cell culture supernatants.

Brand:  Invitrogen™ 88-7620-86

Product Code. 15501177

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

The Human Interleukin 15 (IL-15) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-15. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human IL-15 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA)

Specifications

• Assay Sensitivity: 20pg/mL
• For Use With (Application): ELISA
• Storage Requirements: 2°C to 8°C
• Target: IL-15

• Target Species: Human
• Quantity: 10 x 96 Tests
• Synonym: Interleukin-15

For Research Use Only