eBioscience™ Human TGF beta 3, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: eBioscience™

Manufacturer Part Number: 34-8734-82

100UG Human TGF beta 3 Recombinant Protein Carrier-Free

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15578826

Quantity Price
1 £ 1564.3 / 100µg
Estimated Shipment date
from Supplier 23-02-2017
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Description and Specification

Specification

Name TGF beta 3
Purification Method SDS-PAGE and HPLC
Purity >98%
Recombinant Yes
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Regulatory Status RUO
Structural Form E. coli (amino acids Ala 301 - Ser412; Accession # NP_003230.1)
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Gene Symbol Transforming Growth Factor beta 3
Cross Reactivity Human
For Use With (Application) Functional Assays
Molecular Weight 25.0kDa
Quantity 100μg
Source E. coli
Conjugate Unlabeled
Biological Activity The ED50 of this protein, as measured by inhibition of IL-4-induced proliferation of mouse HT-2 cells, is less than or equal to 0.05ng/mL, which corresponds to a specific activity of greater than 2 x 107 Units/mg.
Product Type Recombinant Protein, Carrier-Free
Formulation Lyophilized from 0.1% TFA.

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.

The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.