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Mouse LIF, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8521-62

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15537546

  • £62.79 / 5µg


Estimated Shipment date
from Supplier 24-07-2018
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Description

Description

Leukemia inhibitory factor (LIF) is a 20kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. Description: Leukemia inhibitory factor (LIF) is a 20kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFalpha and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific. Applications Reported: Recombinant mouse LIF is biologically active. Applications Tested: Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 10e6 - 2.5 x 10e6 Units/mg. Source: E. coli derived Ser24-Phe203 accession number NM_008501. Bioactivity: Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 10e6 - 2.5 x 10e6 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 20 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

P09056
Cytokine Bioassays
PBS with 0.05% Tween 20, 1% BSA and no preservative
20kDa
SDS-PAGE
5μg
-80°C
E. coli derived Ser24-Phe203 accession number NM_008501
Less than 0.01ng/μg cytokine as determined by the LAL assay
Lif
Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 106 - 2.5 x 106 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration.
Mouse
Ms
Recombinant
0.1mg/mL
Liquid
16878
Mouse LIF
Affinity chromatography
E. coli
RUO
Recombinant
Mouse LIF
Control, Functional Assay
Recombinant Protein
Unlabeled
E. coli
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