Invitrogen™ eBioscience™ Mouse LIF, Recombinant Protein
Mouse Recombinant Protein
Brand: Invitrogen™ 14-8521-62
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionLeukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.
|Store at less than or equal to -70°C.|
|E. coli derived Ser24-Phe203 accession number NM_008501|
|leukocyte inhibitory factor, Leukemia inhibitory factor|
|Sterile liquid; phosphate-buffered saline with 0.05% Tween-20 and 1% BSA, 0.22μm filtered.|
|Less than 0.01ng/μg cytokine as determined by the LAL assay|
|Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 106 - 2.5 x 106 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration.|
For Research Use Only.