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Invitrogen™ IL-12/IL-23 p40 Human ELISA Kit

For the quantitative detection of human IL-12/IL-23 p40.

Brand:  Invitrogen™ BMS2013

300.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15521487

  • £451.00 / 96 tests
Estimated Shipment: 23-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-12/IL-23 p40
Biotin-Conjugate anti-human IL-12/IL-23 p40 monoclonal antibody
Streptavidin-HRP
Human IL-12/IL-23 p40 Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

The Human Interleukin-12/Interleukin-23 p40 subunit (Hu IL-12/IL-23 p40) ELISA quantitates Hu IL-12/IL-23 p40 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-12/IL-23 p40. Principle of the method The Human IL-12/IL-23 p40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
Specifications

Specifications

P29460,Q9NPF7
<10 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2,IL-23,IL-23A,IL23P19,P19,SGRF
4.7%
HRP
RUO
2°C to 8°C
Human
4 hr. 10 min.
31.3-2,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3593, 51561
6.4%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Interleukin-12 subunit beta precursor
1 hr. 20 min.
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