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Invitrogen™ IL-10 Human ELISA Kit

For the quantitative detection of human IL-10

Brand:  Invitrogen™ BMS215-2

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15591687

  • £377.00 / 96 tests


In stock, ready to ship: 16
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-10
Biotin-Conjugate anti-human IL-10 monoclonal antibody
Streptavidin-HRP
Human IL-10 Standard lyophilized, 400pg/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

Description

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
Specifications

Specifications

P22301
HRP
Biotin
3586
ELISA Kit
1.0 pg/mL
Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL
1 hr. 20 min.
Human
TGIF, MCSF III, CSIF, B TCGF
3.2%
Human
Colorimetric Microplate Reader
3.15-200 pg/mL
ELISA
Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution
RUO
50 μL
2°C to 8°C
3 hr. 10 min.
CSIF,GVHDS,IL-10A,TGIF,IL10
5.6%
96 Tests
Supernatant
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For Research Use Only