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Invitrogen™ IL-10 Human ELISA Kit

For the quantitative detection of human IL-10

£451.00 - £3070.00

Specifications

Accession Number P22301
Assay Range 3.15-200 pg/mL
Assay Sensitivity 1.0 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-10
Biotin-Conjugate anti-human IL-10 monoclonal antibody
Streptavidin-HRP
Human IL-10 Standard lyophilized, 400pg/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

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Invitrogen™
BMS215-2
96 Tests
£451.00
96 tests
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Estimated Shipment: 09-04-2024
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15511697
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Invitrogen™
BMS215-2TEN
10 x 96 Tests
£3070.00
Pack of 10
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Estimated Shipment: 22-04-2024
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Description

Description

The Human Interleukin-10 (Hu IL-10) ELISA quantitates Hu IL-10 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10. Principle of the method The Human IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P22301
1.0 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CSIF,GVHDS,IL-10A,TGIF,IL10
3.2%
HRP
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
TGIF, MCSF III, CSIF, B TCGF
1 hr. 20 min.
3.15-200 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3586
5.6%
Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, SAV-HRP, Controls, Chromogen, Chromogen, Stop Solution
RUO
2°C to 8°C
Human
3 hr. 10 min.
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Product Certifications

For Research Use Only